efeB [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

elemental iron uptake system, heme peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by EfeO-EfeU, peroxide detoxification under microaerobic conditions

Locus: BSU38260

Isoelectric point: 8.00

Molecular weight: 45.00 kDa

Function: ferrous iron conversion

Product: heme peroxidase in elemental iron uptake

Essential: no

E.C.: 1.11.1.-

Synonyms: ipa-29d, ywbN

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Acquisition of iron → Elemental iron transport system
• Metabolism → Additional metabolic pathways → Iron metabolism → Elemental iron transport system
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Secreted proteins
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• acquisition of iron, iron metabolism, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against oxidative and electrophile stress, membrane proteins

This gene is a member of the following regulons

• Fur regulon, SigM regulon, SigW regulon, SigX regulon

Gene

Coordinates on the chromosome (coding sequence): 3,926,682 -> 3,927,932

The protein

Catalyzed reaction/ biological activity

• oxidizes ferrous iron to ferric iron for uptake by EfeO-EfeU PubMed
• eliminates reactive oxygen species that accumulate in the presence of ferrous iron PubMed
• peroxide detoxification under microaerobic conditions PubMed

Protein family

• DyP-type peroxidase family (according to Swiss-Prot)

Cofactors

• protoheme IX PubMed

Effectors of protein activity

• activity is increased in the presence of EfeO due to the displacement of ferric iron PubMed

Structure

• 2Y4F (from E. coli, 38% identity)

Localization

• extracellular, secreted by the TatAY-TatCY complex PubMed
• TatAY-TatCY-dependent export of EfeB requires a functional WprA PubMed
• forms a membrane-bound complex with EfeU and EfeO for iron uptake PubMed
• attached to the membrane via EfeU PubMed

Interactions

• EfeO-EfeU-EfeB PubMed

Expression and Regulation

Operon

• efeU-efeO-efeB PubMed
• efeB-ywbO PubMed

Sigma factor

• efeU: SigA PubMed
• efeB: SigM PubMed, SigX, SigW PubMed

Regulation

• induced upon iron starvation (Fur) PubMed

Regulatory mechanism

• Fur: transcription repression PubMed

Labs working on this gene/protein

• Jan Maarten van Dijl, Groningen, Netherlands

References

Reviews

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Original publications

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