efeB

elemental iron uptake system, heme peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|EfeO]-[protein|3BED98E311EF380AE77327C7F0A83254805089C0|EfeU], peroxide detoxification under microaerobic conditions

locus: BSU_38260

pI: 8.64

mw: 45.53 kDa

protein length: 416 aa

gene length: 1251 bp

function: ferrous iron conversion

product: heme peroxidase in elemental iron uptake

essential: no

ec: 1.11.1.-

synonyms: ipa-29d,ywbN

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.2|Elemental iron transport system]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.5|Elemental iron transport system]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,926,682 3,927,932

The protein

Catalyzed reaction/ biological activity

• oxidizes ferrous iron to ferric iron for uptake by [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|EfeO]-[protein|3BED98E311EF380AE77327C7F0A83254805089C0|EfeU] [Pubmed|23764491]
• eliminates reactive oxygen species that accumulate in the presence of ferrous iron [Pubmed|23764491]
• peroxide detoxification under microaerobic conditions [Pubmed|23764491]

Protein family

• DyP-type peroxidase family (single member, according to UniProt)

[SW|Cofactors]

• protoheme IX [Pubmed|23764491]

Effectors of protein activity

• activity is increased in the presence of [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|EfeO] due to the displacement of ferric iron [Pubmed|23764491]

Structure

• [PDB|2Y4F] (from ''E. coli'', 38% identity)

[SW|Localization]

• extracellular, secreted by the [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY] complex [Pubmed|15554971]
• [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY]-dependent export of [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|EfeB] requires a functional [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|WprA] [Pubmed|23180473]
• forms a membrane-bound complex with [protein|3BED98E311EF380AE77327C7F0A83254805089C0|EfeU] and [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|EfeO] for iron uptake [Pubmed|23180473,23764491]
• attached to the membrane via [protein|3BED98E311EF380AE77327C7F0A83254805089C0|EfeU] [Pubmed|23764491]

Expression and Regulation

Operons

• genes: [gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23764491], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9683469], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]

• genes: [gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]-[gene|65FC8F21D8927C231B55E8693BD29F052A830AF6|ywbO]
  description: [Pubmed|18179421]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9683469], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced upon iron starvation ([protein|search|Fur]) [Pubmed|12354229]

Biological materials

Mutant

• MGNA-B228 (ywbN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1227 NBRP B. subtilis, Japan]
• BKE38260 ([gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTTACAAAACTCCT, downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC
• BKK38260 ([gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTTACAAAACTCCT, downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC

labs

• [SW|Jan Maarten van Dijl], Groningen, Netherlands

References

Reviews

• 24140208

Original publications

• 16672620,19180538,12354229,19383693,9353933,9683469,15554971,21479178,18179421,22923395,23180473,23560556,23764491,23820555,24620988,18931290,26239117,27795321,29133393