spoIIAA [2019-06-21 10:38:43]

anti-anti-SigF

Locus: BSU23470

Isoelectric point: 5.86

Molecular weight: 12.85 kDa

Protein length: 117 aa

Gene length: 354 bp

Function: control of SigF activity

Product: anti-anti-SigF

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 2,444,645 2,444,998

The protein

Protein family

• anti-sigma-factor antagonist family (according to Swiss-Prot)

Modification

• phosphorylation on (Ser-58 OR Ser-59) by SpoIIABPubMed, PubMed

Structure

• 1TIL (complex with SpoIIAB, Geobacillus stearothermophilus) PubMed
• 1AUZ (NMR)

Expression and Regulation

Operons

• Genes: spoIIAA-spoIIAB-sigF
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, in SigF regulon
  • SigG: sigma factor, in SigG regulon
  • SigH: sigma factor, in SigH regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: repression, in SinR regulon

  Regulation

  • spoIIAA: expressed early during sporulation

• Genes: dacF-spoIIAA-spoIIAB-sigF
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, in SigG regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • spoIIAA: expressed early during sporulation
  • strongly repressed in the presence of salt (1.2 M NaCl) PubMed

Biological materials

Mutant

• BKE23470 (spoIIAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTCATTCCTCCTTGAT, downstream forward: _UP4_CTCCTGACACTGGGGGTGGC
• BKK23470 (spoIIAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTCATTCCTCCTTGAT, downstream forward: _UP4_CTCCTGACACTGGGGGTGGC

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Charles Moran, Emory University, NC, USA homepage

References

Modeling of SigF activation

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Original Publications

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