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exoA [Wed Jun 29 2016 13:04:45 GMT+0200 (CEST)]
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exoA [Wed Jun 29 2016 13:04:45 GMT+0200 (CEST)]

apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance
locus
BSU40880
pI
4.00
mw
29.00 kDa
function
repair of oxidative DNA damage in spores
product
apurinic/apyrimidinic endonuclease
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|sporulation proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigG regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,197,780 -> 4,198,538

    Phenotypes of a mutant

  • an ''[protein|search|exoA] [protein|search|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[protein|search|disA]'' [Pubmed|24244006]
  • an ''[protein|search|exoA] [protein|search|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]

    • The protein

    Catalyzed reaction/ biological activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)

    • Protein family

  • DNA repair enzymes AP/exoA family (according to Swiss-Prot)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|exoA]-[protein|search|ccpB]'' (according to [http://dbtbs.hgc.jp/COG/prom/exoA-ccpA.html DBTBS])

    • [SW|Sigma factor]

  • [protein|search|SigG] [Pubmed|16237020]

    • Regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16237020]

    • Biological materials

    Mutant

  • GP898 (''exoA''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP1503 (''exoA''::''kan'', ''nfo''::''cat''), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 22933559

    • Original publications

  • 24244006,18203828,16237020,19930460,10540738,21441501,24123749,24123749,24914186