exoA

apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance

Locus: BSU_40880

Isoelectric point: 4.59

Molecular weight: 29.10 kDa

Protein length: 252 aa

Gene length: 759 bp

Function: repair of oxidative DNA damage in spores

Product: apurinic/apyrimidinic endonuclease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → A/P endonucleases
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 4,197,780 4,198,538

Phenotypes of a mutant

• an exoR nfo double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of disA PubMed
• an exoR nfo double mutant is sensitive to radiation PubMed
• sensitive to blue light-induced DNA damage PubMed
• reduced resistance towards electron beams PubMed

The protein

Catalyzed reaction/ biological activity

• Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to UniProt)

Protein family

• DNA repair enzymes AP/ExoA family (single member, according to UniProt)

Structure

• 5CFE PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: exoA-ccpB
  Description: (according to DBTBS) null

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

Biological materials

Mutant

• GP898 (ΔexoA::kan), available in Jörg Stülke's lab PubMed
• GP1503 (ΔexoA::kan, nfo::cat), available in Jörg Stülke's lab PubMed
• BKE40880 (ΔexoA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCATCCTCCCCCCT, downstream forward: _UP4_TGATGAGATAGCAGTAAGGA
• BKK40880 (ΔexoA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCATCCTCCCCCCT, downstream forward: _UP4_TGATGAGATAGCAGTAAGGA

References

Reviews

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Original publications

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