msrA [2017-8-11 16:0:3]

peptide methionine sulfoxide reductase

locus: BSU21690

pI: 5.71

mw: 20.04 kDa

protein length: 177 aa

gene length: 531 bp

function: regeneration of methionine and restoration of protein function after oxidative damage

product: peptide methionine sulfoxide reductase

essential: no

ec: 1.8.4.11

synonyms: yppP

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,287,529 → 2,288,062

The protein

Catalyzed reaction/ biological activity

• Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (S)-S-oxide + thioredoxin (according to Swiss-Prot)

Structure

• [PDB|2GT3] (from E. coli, 45% identity) [pubmed|17157315]

Expression and Regulation

Operons

• genes: [gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]
  description: [Pubmed|18662407]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18662407,17289755], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, oxidized [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] binds [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|RpoA] and enhances [protein|389245FDE13226D65DF117E28B5DA346DC856860|MsrA]-[protein|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|MsrB] operon transcription [Pubmed|18662407], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by oxidative stress ([protein|search|Spx])

Biological materials

Mutant

• MGNA-A894 (yppP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/894 NBRP B. subtilis, Japan]

References

• 18662407,17157315