msrA

peptide methionine sulfoxide reductase

Locus: BSU_21690

Isoelectric point: 5.71

Molecular weight: 20.04 kDa

Protein length: 177 aa

Gene length: 534 bp

Function: regeneration of methionine and restoration of protein function after oxidative damage

Product: peptide methionine sulfoxide reductase

Essential: no

E.C.: 1.8.4.11

Synonyms: yppP

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 2,287,529 2,288,062

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + H₂O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein](according to UniProt)
• [thioredoxin]-disulfide + H₂O + L-methionine --> [thioredoxin]-dithiol + L-methionine (S)-S-oxide (according to UniProt)

Protein family

• MsrA Met sulfoxide reductase family (single member, according to UniProt)

Structure

• 2GT3 (from E. coli, 45% identity) PubMed

Expression and Regulation

Operons

• Genes: msrA-msrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spx: activation, oxidized Spx binds RpoA and enhances MsrA-MsrB operon transcription PubMed, in Spx regulon

  Regulation

  • induced by oxidative stress (Spx)

Biological materials

Mutant

• MGNA-A894 (yppP::erm), available at the NBRP B. subtilis, Japan
• BKE21690 (msrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA
• BKK21690 (msrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA

References

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