SubtiBank SubtiBank
msrA
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

msrA

peptide methionine sulfoxide reductase
Locus
BSU_21690
Isoelectric point
5.71
Molecular weight
20.04 kDa
Protein length
177 aa Sequence Blast
Gene length
534 bp Sequence Blast
Function
regeneration of methionine and restoration of protein function after oxidative damage
Product
peptide methionine sulfoxide reductase
Essential
no
E.C.
1.8.4.11
Synonyms
yppP

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
2,287,529 2,288,062

The protein

Catalyzed reaction/ biological activity

  • [thioredoxin]-disulfide + H2O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein](according to UniProt)
  • [thioredoxin]-disulfide + H2O + L-methionine --> [thioredoxin]-dithiol + L-methionine (S)-S-oxide (according to UniProt)
  • Protein family

  • MsrA Met sulfoxide reductase family (single member, according to UniProt)
  • Structure

  • 2GT3 (from E. coli, 45% identity) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Spx: activation, oxidized Spx binds RpoA and enhances MsrA-MsrB operon transcription PubMed, in Spx regulon
  • Regulation

  • induced by oxidative stress (Spx)
  • view in new tab

    Biological materials

    Mutant

  • MGNA-A894 (yppP::erm), available at the NBRP B. subtilis, Japan
  • BKE21690 (msrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA
  • BKK21690 (msrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA
  • References

    Loading