ywdK [2019-07-15 09:37:03]

unknown

locus: BSU_37930

pI: 9.43

mw: 11.89 kDa

protein length: 123 aa

gene length: 372 bp

function: unknown

product: unknown

essential: no

synonyms: ipa-61d

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,894,030 3,894,401

The protein

Protein family

• UPF0382 family (according to Swiss-Prot)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F267C75289D19D039F6E97F5DABCDFF0B4E4B8FA|ywdI]-[gene|BA4D8EF5B09345E94EEEEB936C803B71340DC5EE|ywdJ]-[gene|386D4F640E987E3752BEBE57BF25DF487D12EFCE|ywdK]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

Biological materials

Mutant

• MGNA-B237 (ywdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1236 NBRP B. subtilis, Japan]
• BKE37930 ([gene|386D4F640E987E3752BEBE57BF25DF487D12EFCE|ywdK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCTCTCCCGCCTTT, downstream forward: _UP4_TAAACAAAAAAGCCAATCCT
• BKK37930 ([gene|386D4F640E987E3752BEBE57BF25DF487D12EFCE|ywdK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCTCTCCCGCCTTT, downstream forward: _UP4_TAAACAAAAAAGCCAATCCT

References

• 12823818,25755103