SubtiBank SubtiBank
araD
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araD

L-ribulose-phosphate 4-epimerase
Locus
BSU_28780
Isoelectric point
5.24
Molecular weight
25.54 kDa
Protein length
229 aa Sequence Blast
Gene length
690 bp Sequence Blast
Function
arabinose utilization
Product
L-ribulose-phosphate 4-epimerase
Essential
no
E.C.
5.1.3.4
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol

    • Gene

    Coordinates
    2,944,983 2,945,672

    The protein

    Catalyzed reaction/ biological activity

  • L-ribulose 5-phosphate --> D-xylulose 5-phosphate (according to UniProt)

    • Protein family

  • aldolase class II family (with MtnB, according to UniProt)

    • Structure

  • 1K0W (from Escherichia coli, 59% identity, 74% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    araA-araB-araD-araL-araM-araN-araP-araQ-abfA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

    • Regulation

  • induced by arabinose (AraR) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • BKE28780 (araD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAAGCATGCTCCATCACC, downstream forward: _UP4_CATTTTTATCGAAAACACGG
  • BKK28780 (araD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAAGCATGCTCCATCACC, downstream forward: _UP4_CATTTTTATCGAAAACACGG

    • References

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