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minD
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minD

cell division inhibitor (septum placement), part of the Min system (with Z ring placement), required for the disassembly of polar Z rings after cell division
Locus
BSU_27990
Isoelectric point
4.98
Molecular weight
29.26 kDa
Protein length
268 aa Sequence Blast
Gene length
807 bp Sequence Blast
Function
septum placement
Product
cell division inhibitor
Essential
no
Synonyms
divIVB1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell division → The Min system
  • Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,857,776 2,858,582

    Phenotypes of a mutant

  • produces minicells PubMed
  • a whiA minC-minD mutant grows poorly, and the cells are filamentous PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle PubMed
  • required for oriC placement during spore development PubMed

    • Protein family

  • ParA family (with FlhG and ParA, according to UniProt)

    • Structure

  • 4V03 (from Aquifex aeolicus, 51% identity) PubMed

    • Localization

  • polar/ septal at the cell membrane PubMed
  • membrane binding/ polar localization depends on the proton motive force PubMed

    • Expression and Regulation

    Operons

    Genes
    mreB-mreC-mreD-minC-minD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, promoter p1, upstream of Maf PubMed, in SigA regulon
  • SigM: sigma factor, promoter p2, within Maf PubMed, in SigM regulon
  • SigH: sigma factor, promoter p4, upstream of MinC PubMed, in SigH regulon

    • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

    • Regulation

  • constitutively expressed PubMed
    • view in new tab

    Genes
    minC-minD
    Description
    PubMed

    Regulation

  • constitutively expressed PubMed
    • view in new tab

    Genes
    maf-radC-mreB-mreC-mreD-minC-minD
    Description
    PubMed

    Sigma factors

  • SigM: sigma factor, promoter p2 within Maf PubMed, in SigM regulon
  • SigA: sigma factor, promoter p1, upstream of Maf PubMed, in SigA regulon

    • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

    • Regulation

  • constitutively expressed PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE27990 (minD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA
  • BKK27990 (minD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

    Reviews

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    Original Publications

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