yrvM

2-methylthio-N6-threonylcarbamoyladenosine cyclase, catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )

Locus: BSU_27540

Isoelectric point: 8.19

Molecular weight: 28.02 kDa

Protein length: 254 aa

Gene length: 765 bp

Function: tRNA modification

Product: 2-methylthio-N6-threonylcarbamoyladenosine cyclase

Essential: no

Synonyms: yrvM

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 2,813,643 2,814,407

The protein

Catalyzed reaction/ biological activity

• catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A ) PubMed

Protein family

• HesA/MoeB/ThiF family (with MoeB and ThiF, according to UniProt)

Structure

• 4D7A (from E. coli, 40% identity) PubMed

Expression and Regulation

Operons

• Genes: bsrA-yrvM
  Description: PubMed

Biological materials

Mutant

• MGNA-A826 (yrvM::erm), available at the NBRP B. subtilis, Japan
• BKE27540 (yrvM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAACCTCACTCCACATT, downstream forward: _UP4_TAATGCATAAAAACAGCCTT
• BKK27540 (yrvM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAACCTCACTCCACATT, downstream forward: _UP4_TAATGCATAAAAACAGCCTT

References

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