bdhA [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

acetoine/ butanediol dehydrogenase

locus: BSU06240

pI: 4.00

mw: 37.00 kDa

function: overflow metabolism, fermentation

product: acetoine/ butanediol dehydrogenase

essential: no

synonyms: ydjL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon]

Gene

Coordinates on the chromosome (coding sequence): 677,911 -> 678,951

The protein

Catalyzed reaction/ biological activity

• formation of butanediol from acetoin [Pubmed|18820069]

Modification

• phosphorylated on Arg-13 [Pubmed|22517742]

Structure

• [PDB|4EJ6] (from ''Sinorhizobium meliloti'', 33% identity)

Expression and Regulation

Operon

• ''bdhA'' (according to [http://dbtbs.hgc.jp/COG/prom/ydjL.html DBTBS])

Regulatory mechanism

• [protein|search|AbrB]: transcription repression [Pubmed|20817675]

Additional information

• The mRNA has a long (268 nt) untranslated leader region [PubMed|23576037]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Biological materials

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 18820069,22361854,22517742,22178965,20817675,23576037,15378759,24608678,26385762,26825987