infB [Mon Aug 31 2015 13:54:21 GMT+0200 (CEST)]

translation initiation factor IF-2

locus: BSU16630

pI: 5.19

mw: 78.00 kDa

function: translation

product: initiation factor IF-2

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|stringent response]

Gene

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Initiation of translation

Protein family

• IF-2 family (according to Swiss-Prot)

[SW|Domains]

• Nucleotide-binding domain that competitively binds GTP or ppGpp [Pubmed|16968770]

Effectors of protein activity

• GTP and ppGpp [Pubmed|16968770]

Structure

• [PDB|1D1N] (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), [PDB|1Z9B] (C1-subdomain, Geobacillus stearothermophilus)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|ylxS]-[protein|search|nusA]-[protein|search|ylxR]-[protein|search|ylxQ]-[protein|search|infB]-[protein|search|ylxP]-[protein|search|rbfA]'' [Pubmed|8491709]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8491709]

Regulation

• [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
• Interaction with ppGpp interferes with IF2-dependent initiation complex formation, severely inhibits initiation dipeptide formation, and blocks the initiation step of translation.[Pubmed|16968770]

References

• 11948165,8491709,16968770