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prs [2019-04-15 18:37:33]
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prs [2019-04-15 18:37:33]

phosphoribosylpyrophosphate synthetase, universally conserved protein
locus
BSU00510
pI
5.89
mw
34.71 kDa
protein length
317 aa Sequence Blast
gene length
954 bp Sequence Blast
function
phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
product
phosphoribosylpyrophosphate synthetase
essential
yes
ec
2.7.6.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

    • Gene

    Coordinates
    57,745 58,698

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

    • Protein family

  • Ribose-phosphate pyrophosphokinase family (according to UniProt)

    • Effectors of protein activity

  • subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]

    • Structure

  • [PDB|1IBS]
  • [PDB|1DKR]
  • [PDB|1DKU]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • Additional information

  • [SW|universally conserved protein]

    • Expression and Regulation

    Operons

    genes
    [gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|8522540], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
    • view in new tab

    Biological materials

    Expression vectors

  • N-terminal Strep-tag, expression in ''E. coli'', in [SW|pGP172]: pGP2541, available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 28031352

    • Original publications

  • 2169413,8522540,12837783,12837784,19446032,22083279