phosphoribosylpyrophosphate synthetase, universally conserved protein
function
phosphoribosylpyrophosphate synthesis
(biosynthesis of histidine)
product
phosphoribosylpyrophosphate synthetase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine][category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides][category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids], [SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes], [SW|universally conserved proteins]This gene is a member of the following [SW|regulons]
[SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
57,745 -> 58,698
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
ATP D-ribose 5-phosphate = AMP 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot) Protein family
ribose-phosphate pyrophosphokinase family (according to Swiss-Prot)Effectors of protein activity
subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]Structure
[PDB|1IBS] [PDB|1DKR][PDB|1DKU][SW|Localization]
cytoplasm (according to Swiss-Prot)[SW|Interactions]
[protein|search|PurR]-[protein|search|Prs]Additional information
[SW|universally conserved protein]Expression and Regulation
Operon
''[protein|search|gcaD]-[protein|search|prs]-[protein|search|ctc]'' [Pubmed|8522540][SW|Sigma factor]
[protein|search|SigA] [Pubmed|8522540]Regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]Biological materials
Expression vector
N-terminal Strep-tag, expression in ''E. coli'', in [SW|pGP172]: pGP2541, available in [SW|Jörg Stülke]'s labReferences
2169413,8522540,12837783,12837784,19446032,22083279 
