prs

phosphoribosylpyrophosphate synthetase, universally conserved protein

locus: BSU_00510

pI: 5.89

mw: 34.71 kDa

protein length: 317 aa

gene length: 954 bp

function: phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)

product: phosphoribosylpyrophosphate synthetase

essential: yes

ec: 2.7.6.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

Gene

Coordinates: 57,745 58,698

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + D-ribose 5-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + AMP + H⁺ (according to UniProt)

Protein family

• Ribose-phosphate pyrophosphokinase family (single member, according to UniProt)

Effectors of protein activity

• subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]

Structure

• [PDB|1IBS]
• [PDB|1DKR]
• [PDB|1DKU]

[SW|Localization]

• cytoplasm (according to UniProt)

Additional information

• [SW|universally conserved protein]

Expression and Regulation

Operons

• genes: [gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|8522540], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

Biological materials

Expression vectors

• N-terminal Strep-tag, expression in ''E. coli'', in [SW|pGP172]: pGP2541, available in [SW|Jörg Stülke]'s lab

References

Reviews

• 28031352

Original publications

• 2169413,8522540,12837783,12837784,19446032,22083279