SubtiBank SubtiBank
lepA
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lepA

elongation factor 4, GTP-binding protein
Locus
BSU_25510
Isoelectric point
4.98
Molecular weight
68.41 kDa
Protein length
612 aa Sequence Blast
Gene length
1839 bp Sequence Blast
Function
translation elongation
Product
elongation factor 4
Essential
no
Synonyms
yqxB,yqeQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → GTP-binding proteins

    • Gene

    Coordinates
    2,630,910 2,632,748

    The protein

    Catalyzed reaction/ biological activity

  • GTP + H2O --> GDP + H+ + phosphate (according to UniProt)

    • Protein family

  • TRAFAC class translation factor GTPase superfamily (according to UniProt)
  • Classic translation factor GTPase family (according to UniProt)

    • Domains

  • tr-type G domain (aa 12-194) (according to UniProt)

    • Structure

  • 4QJT (LepA bound to the Thermus thermophilus ribosome, 62% identity) PubMed

    • Localization

  • cytoplasm (Homogeneous) PubMed

    • Expression and Regulation

    Operons

    Genes
    lepA-hemN
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Additional information

  • There is a terminator between 'lepA' and 'hemN' with only little readthrough
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    Biological materials

    Mutant

  • BKE25510 (lepA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATCTATCACTCCTACT, downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG
  • BKK25510 (lepA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATCTATCACTCCTACT, downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG

    • References

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