SubtiBank SubtiBank
ycbF
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ycbF

glucarate dehydratase
Locus
BSU_02490
Isoelectric point
5.59
Molecular weight
50.62 kDa
Protein length
455 aa Sequence Blast
Gene length
1368 bp Sequence Blast
Function
glucarate utilization
Product
glucarate dehydratase
Essential
no
E.C.
4.2.1.40
Synonyms
gudD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucarate/galactarate

    • Gene

    Coordinates
    271,800 273,167

    The protein

    Catalyzed reaction/ biological activity

  • D-glucarate --> 5-dehydro-4-deoxy-D-glucarate + H2O (according to UniProt)

    • Protein family

  • mandelate racemase/muconate lactonizing enzyme family (according to UniProt)

    • Structure

  • 1EC7 (from E. coli, 73% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
    Description
    PubMed

    Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon

    • Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed
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    Biological materials

    Mutant

  • MGNA-C029 (ycbF::erm), available at the NBRP B. subtilis, Japan
  • BKE02490 (ycbF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCATTTTCCTCCCTTTA, downstream forward: _UP4_TAAGTTTGATTGTAAATATT
  • BKK02490 (ycbF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCATTTTCCTCCCTTTA, downstream forward: _UP4_TAAGTTTGATTGTAAATATT

    • References

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