sigA

[SW|RNA polymerase] major [SW|sigma factor] SigA

locus: BSU_25200

pI: 4.63

mw: 42.80 kDa

protein length: 371 aa

gene length: 1116 bp

function: transcription

product: [SW|RNA polymerase] major [SW|sigma factor] SigA

essential: yes

synonyms: rpoD,crsA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,600,214 2,601,329

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Protein family

• [SW|Sigma-70 factor family] (according to UniProt)

Modification

• phosphorylated on Arg-365 [Pubmed|31221751]

Structure

• [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 69% identity) [Pubmed|19903881]

[SW|Localization]

• cytoplasm [Pubmed|25313396]

Expression and Regulation

Operons

• genes: [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
  description: [Pubmed|3919021]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|3919021], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]

• genes: [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
  description: [Pubmed|3298998]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

  additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]

labs

• [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 22210308,24308327

Original publications

• 12486038,10556308,8107087,14762002,7918363,8071196,7630711,9185571,9287022,14679239,14976210,19735077,2997585,9658000,3127379,10438769,11535782,7599136,9144176,19680289,20525796,22333917,20935043,20817769,23875654,25112476,26582911,19903881,27977677,28539362,28648455,29363854,31221751,32127479