bglP [Mon Aug 31 2015 13:57:37 GMT+0200 (CEST)]

trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA of the PTS

locus: BSU39270

pI: 6.81

mw: 64.00 kDa

function: beta-glucoside uptake and phosphorylation,
control of LicT activity

product: trigger enzyme: beta-glucoside-specific
phosphotransferase system, EIIBCA

essential: no

ec: 2.7.1.69

synonyms: sytA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

syns: sytA

[SW|Categories] containing this gene/protein

• [SW|phosphotransferase systems], [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|membrane proteins], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|LicT regulon]

The protein

Catalyzed reaction/ biological activity

• Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

Protein family

• [SW|PTS] permease, sucrose permease (Scr) family [Pubmed|10627040]

[SW|Localization]

• cell membrane [Pubmed|23475962]

[SW|Interactions]

• [protein|search|BglP]-[PtsH|search|HPr], [protein|search|BglP]-[protein|search|LicT]

Expression and Regulation

Operon

• ''[protein|search|bglP]-[protein|search|bglH]-[protein|search|yxiE]'' [Pubmed|7883710]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|7559347]

Regulation

• repressed by glucose ([protein|search|CcpA], [protein|search|LicT]) [Pubmed|7559347]
• induced by salicin ([protein|search|LicT]) [Pubmed|7883710]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression, ([protein|search|CcpA] binding site overlaps -35 region) [Pubmed|7559347]
• [protein|search|LicT]-dependent [SW|RNA switch] (antitermination, lack of [protein|search|LicT]-dependent antitermination in the presence of gucose due to the requirement of [protein|search|LicT] to be phosphorylated by [PtsH|search| HPr] [Pubmed|8626332]

Biological materials

Mutant

• GP475 (erm), available in the [SW|Stülke] lab

Expression vector

• pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Stülke] lab,
• pGP1300 (expression of ''[protein|search|bglP]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Stülke] lab

lacZ fusion

• pGP600 (in [protein|search|pAC6]), available in [SW|Stülke] lab

References

• 16672620,20525796,10627040,7883710,8626332,7559347,22900538,23475962