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bglP [2017-8-11 16:0:3]
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bglP [2017-8-11 16:0:3]

trigger enzyme,beta-glucoside uptake and control of LicT activity
Locus
BSU39270
Isoelectric point
6.81
Molecular weight
64.52 kDa
Protein length
609 aa Sequence Blast
Gene length
1827 bp Sequence Blast
Function
beta-glucoside uptake and phosphorylation, control of LicT activity
Product
trigger enzyme
Essential
no
E.C.
2.7.1.69
Synonyms
sytA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of beta-glucosides
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    4,033,778 → 4,035,607

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

    • Protein family

  • PTS permease, sucrose permease (Scr) family PubMed

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    bglP-bglH-yxiE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, (CcpA binding site overlaps -35 region) PubMed, in CcpA regulon
  • LicT: anti-termination, via LicT-dependent RNA switch, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HprK, in LicT regulon

    • Regulation

  • induced by salicin (LicT) PubMed

    • Additional information

  • An [ncRNA|search|antisense RNA] is predicted for 'bglP' PubMed
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    Biological materials

    Mutant

  • GP475 (erm), available in the Stülke lab

    • Expression vector

  • pGP1290 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab,
  • pGP1300 (expression of bglP in B. subtilis, in pBQ200), available in Stülke lab

    • LacZ fusion

  • pGP600 (in pAC6), available in Stülke lab

    • References

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