tcyL

cystine ABC transporter (permease)

Locus: BSU_29360

Isoelectric point: 9.30

Molecular weight: 26.09 kDa

Protein length: 239 aa

Gene length: 720 bp

Function: cystine uptake

Product: cystine ABC transporter (permease)

Essential: no

Synonyms: ytmL

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,005,859 3,006,578

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Paralogous protein(s)

• YxeN

Domains

• ABC transmembrane type-1 domain (aa 21-216) (according to UniProt)

Structure

• 4YMS (amino acid transporter from Caldanaerobacter subterraneus, 31% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

  Regulation

  • induced in the presence of methionine and taurine PubMed

Biological materials

Mutant

• MGNA-A172 (ytmL::erm), available at the NBRP B. subtilis, Japan
• 1A948 ( tcyL::kan), PubMed, available at BGSC
• BKE29360 (tcyL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACTGCTCCCTCTCTTT, downstream forward: _UP4_TGATATATTTCTTCGGATGA
• BKK29360 (tcyL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACTGCTCCCTCTCTTT, downstream forward: _UP4_TGATATATTTCTTCGGATGA

References

Loading