guaC [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

GMP reductase

Locus: BSU32130

Isoelectric point: 6.08

Molecular weight: 35.00 kDa

Function: purine salvage and interconversion

Product: GMP reductase

Essential: no

E.C.: 1.7.1.7

Synonyms: yumD

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Purine salvage and interconversion

Categories containing this gene/protein

• biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

• CodY regulon, PurR regulon

Gene

Coordinates on the chromosome (coding sequence): 3,303,042 -> 3,304,022

The protein

Catalyzed reaction/ biological activity

• Inosine 5'-phosphate + NH₃ + NADP⁺ = guanosine 5'-phosphate + NADPH (according to Swiss-Prot)

Protein family

• GuaC type 2 subfamily (according to Swiss-Prot)

Structure

• 1YPF (from Bacillus anthracis, 84% identity, 91% similarity)

Expression and Regulation

Operon

• guaC PubMed

Regulation

• repressed in the presence of purine nucleotides (PurR) PubMed
• activated during growth in the presence of branched chain amino acids (CodY) PubMed
• expression activated by glucose (3.3 fold) PubMed

Regulatory mechanism

• CodY: transcription activation PubMed
• PurR: transcription repression (molecular inducer: PRPP) PubMed

Additional information

• the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] (the half-life of the mRNA increases from 3 to 20 min) PubMed

References

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