yfhA

[SW|ABC transporter] for the siderophore schizokinen and arthrobactin (permease), works with ATPase [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|YusV]

locus: BSU_08460

pI: 10.26

mw: 35.80 kDa

protein length: 343 aa

gene length: 1032 bp

function: [SW|acquisition of iron]

product: [SW|ABC transporter] for the siderophore schizokinen and arthrobactin (permease)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 921,472 922,503

The protein

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
• [SW|FecCD subfamily] (according to UniProt)

Paralogous protein(s)

• [protein|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|FecE], [protein|EC98685EE0E3CE6CE8DC33409481AE96315279AC|FhuG]

Structure

• [PDB|2NQ2] (complex with [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|YusV]-like protein, from Haemophilus influenzae, 33% identity) [pubmed|17158291]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|81494D4E7E52531802C3C0D0073739DA9375F5DF|yfiZ]-[gene|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|yfhA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

Biological materials

Mutant

• MGNA-C313 (yfhA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2311 NBRP B. subtilis, Japan]
• BKE08460 ([gene|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|yfhA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGCTTCGCCCCCCT, downstream forward: _UP4_TAATCGTTACACCCATTTTC
• BKK08460 ([gene|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|yfhA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGCTTCGCCCCCCT, downstream forward: _UP4_TAATCGTTACACCCATTTTC

References

• 10092453,16672620,12354229,17158291