SubtiBank SubtiBank
levB
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levB

endolevanase
Locus
BSU_34460
Isoelectric point
4.99
Molecular weight
58.81 kDa
Protein length
516 aa Sequence Blast
Gene length
1551 bp Sequence Blast
Function
levan degradation
Product
endolevanase
Essential
no
E.C.
3.2.1.64
Synonyms
yveB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,537,507 3,539,057

The protein

Catalyzed reaction/ biological activity

  • Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain (according to UniProt)
  • Protein family

  • glycosyl hydrolase 32 family (with SacC and SacA, according to UniProt)
  • Structure

  • 4FFF (from Arthrobacter ureafaciens, 42% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • SacY: antitermination, /antitermination via binding to a RNA switch, in SacY regulon
  • Regulation

  • the RNA switch RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B614 (yveB::erm), available at the NBRP B. subtilis, Japan
  • BKE34460 (levB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT, downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG
  • BKK34460 (levB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT, downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG
  • References

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