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mtnB [2019-07-31 13:07:27]
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mtnB [2019-07-31 13:07:27]

methylthioribulose-1-phosphate dehydratase
locus
BSU_13610
pI
5.69
mw
23.34 kDa
protein length
209 aa Sequence Blast
gene length
630 bp Sequence Blast
function
methionine salvage
product
methylthioribulose-1-phosphate dehydratase
essential
no
ec
4.2.1.109
synonyms
ykrY
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

    • Gene

    Coordinates
    1,428,940 1,429,569

    The protein

    Catalyzed reaction/ biological activity

  • S-methyl-5-thio-D-ribulose 1-phosphate = 5-(methylthio)-2,3-dioxopentyl phosphate + H2O (according to Swiss-Prot)

    • Protein family

  • aldolase class II family (with [protein|3859854178E573AE8ACCE6DF85E32C495D316DA1|AraD], according to UniProt)

    • Structure

  • [PDB|2IRP] (from Aquifex aeolicus, 40% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]-[gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]-[gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]-[gene|C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9|mtnD]
    description
    [Pubmed|12022921]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B322 (ykrY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1321 NBRP B. subtilis, Japan]
  • BKE13610 ([gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCTCTCTCTGCCAATTCCC, downstream forward: _UP4_GTTAAATAAAAGGAGGAATT
  • BKK13610 ([gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCTCTCTCTGCCAATTCCC, downstream forward: _UP4_GTTAAATAAAAGGAGGAATT

    • References

  • 12022921,11914366,12107147,15102328,12787499,18039762,28516784