hemD [2019-08-08 10:13:43]

uroporphyrinogen-III synthase

Locus: BSU_28140

Isoelectric point: 5.09

Molecular weight: 28.96 kDa

Protein length: 262 aa

Gene length: 789 bp

Function: heme biosynthesis

Product: uroporphyrinogen-III synthase

Essential: no

E.C.: 4.2.1.75

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme

Gene

Coordinates: 2,875,173 2,875,961

The protein

Catalyzed reaction/ biological activity

• Hydroxymethylbilane = uroporphyrinogen III + H₂O (according to Swiss-Prot)

Protein family

• uroporphyrinogen-III synthase family (single member, according to UniProt)

Expression and Regulation

Operons

• Count: 2
  Genes: hemA-hemX-hemC-hemD-hemB-hemL
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PerR: repression, PubMed, in PerR regulon

  Regulation

  • induced by hydrogen peroxide (PerR) PubMed

Biological materials

Mutant

• BKE28140 (hemD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGTTTTTCCTTTCAACG, downstream forward: _UP4_ATGTCAAGAGAGGAAGAGAG
• BKK28140 (hemD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGTTTTTCCTTTCAACG, downstream forward: _UP4_ATGTCAAGAGAGGAAGAGAG

References

Reviews

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Original Publications

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