SubtiBank SubtiBank
mecA
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mecA

buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK and ComS to the ClpC-ClpP degradation machine (in log phase), inhibits the transcriptional activity of Spo0A-P by direct interaction
Locus
BSU_11520
Isoelectric point
4.21
Molecular weight
25.62 kDa
Protein length
218 aa Sequence Blast
Gene length
657 bp Sequence Blast
Function
control of ComK degradation, regulation of competence
Product
adaptor protein
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → Genetic competence
  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
  • Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence

    • Gene

    Coordinates
    1,229,068 1,229,724

    The protein

    Catalyzed reaction/ biological activity

  • recruits protein for degradation by ClpC-ClpP PubMed
  • inhibits transcription activation of Spo0A-P (in complex with ClpC) PubMed

    • Protein family

  • mecA family (with YpbH, according to UniProt)

    • Paralogous protein(s)

  • YpbH

    • Domains

  • N-terminal domain (1-120): recruitment of protein substrates PubMed
  • C-terminal domain (121-218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed

    • Structure

  • 3PXG (the MecA-ClpC complex) PubMed
  • 3J3U (the MecA-ClpC complex) PubMed
  • 3JTP(C-terminal domain) PubMed

    • Expression and Regulation

    Operons

    Genes
    mecA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • GP813 (spc), GP814 (aphA3) both available in Jörg Stülke's lab
  • BKE11520 (mecA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
  • BKK11520 (mecA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

    Reviews

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    Original Publications

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