lnrL

similar to [SW|ABC transporter] (ATP-binding protein), involved in resistance to linearmycin

locus: BSU_08310

pI: 6.50

mw: 33.95 kDa

protein length: 311 aa

gene length: 936 bp

function: resistance to linearmycin

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 905,816 906,751

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• [SW|ABC transporter domain] (aa 2-232) (according to UniProt)

Structure

• [PDB|4YER] (from Thermotoga maritima, 39% identity)

[SW|Localization]

• membrane associated (via [protein|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|LnrM]-[protein|79BAF2329209C81E8D165F131F9DAADB4A81448A|LnrN]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]-[gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]-[gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN]
  description: [Pubmed|26647299]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]: activation, [Pubmed|26647299], in [regulon|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK regulon]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
  • induced in the presence of linearmycin and other polyenes ([protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]) [pubmed|32973732,28461449]

Biological materials

Mutant

• MGNA-C354 (yfiL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2352 NBRP B. subtilis, Japan]
• BKE08310 ([gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCGTCTCACTCCTTTT, downstream forward: _UP4_TTGCGGGATTGAGGAGGGAC
• BKK08310 ([gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCGTCTCACTCCTTTT, downstream forward: _UP4_TTGCGGGATTGAGGAGGGAC

References

• 10092453,26647299,27766092,28461449,32973732