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yesZ
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yesZ

beta-galactosidase, has transgalactosylation activity
locus
BSU_07080
pI
5.50
mw
73.92 kDa
protein length
663 aa Sequence Blast
gene length
1992 bp Sequence Blast
function
unknown
product
beta-galactosidase
essential
no
ec
3.2.1.23
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

    • Gene

    Coordinates
    774,799 776,790

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to UniProt)

    • Protein family

  • glycosyl hydrolase 42 family (with [protein|DBBA549C2D71CF628E92AC7B659EA276E227D742|GanA], according to UniProt)

    • Structure

  • [PDB|1KWG] (from Thermus thermophilus, 31% identity) [pubmed|12215416]

    • Expression and Regulation

    Operons

    genes
    [gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
    description
    [Pubmed|19651770]

    regulatory mechanism

  • [protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR]: activation, [Pubmed|19651770], in [regulon|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR regulon]

    • regulation

  • induced by pectin ([protein|search|RhgR]) [Pubmed|19651770]
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    Biological materials

    Mutant

  • MGNA-B455 (yesZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1454 NBRP B. subtilis, Japan]
  • BKE07080 ([gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGATACAGTTTTCTCATCA, downstream forward: _UP4_TGATTCTTTGTATCGAATCA
  • BKK07080 ([gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGATACAGTTTTCTCATCA, downstream forward: _UP4_TGATTCTTTGTATCGAATCA

    • References

  • 19651770,17449691,17056685,12215416,30036621