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lytE [2017-11-09 10:26:22]
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lytE [2017-11-09 10:26:22]

cell wall hydrolase (major autolysin) for cell elongation and separation, D,L-endopeptidase-type autolysin
Locus
BSU09420
Isoelectric point
10.71
Molecular weight
37.16 kDa
Protein length
343 aa Sequence Blast
Gene length
1029 bp Sequence Blast
Function
major autolysin, cell elongation and separation
Product
cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Essential
no
E.C.
3.4.-.-
Synonyms
papQ,cwlF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,018,998 → 1,020,002

Phenotypes of a mutant

  • a cwlO lytE mutant is not viable PubMed
  • growth defect at high temperature PubMed
  • inactivation of lytE strongly restores beta-lactam resistance in a sigM mutant by delaying cell lysis PubMed
  • a lytE mutation is synthetically lethal with ftsE and ftsX mutation (due to a lack of autolysin activity) PubMed
  • a lytE mutation increases the cell separation defect of a lytF mutant PubMed
  • cells are thinner (reduced diameter) as compared to the wild type PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
  • Protein family

  • nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
  • Domains

  • contains three N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain PubMed
  • Effectors of protein activity

  • activity requires functional MreB and MreBH PubMed
  • Structure

  • 4XCM (from Thermus thermophilus, 34% identity) PubMed
  • Localization

  • binds the cell wall PubMed
  • localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) PubMed
  • localization to lateral cell wall depends on the interaction with MreBH PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon
  • SigI: sigma factor, PubMed, in SigI regulon
  • Regulatory mechanism

  • WalR: activation, PubMed, in WalR regulon
  • Spo0A: repression, PubMed, in Spo0A regulon
  • Regulation

  • expression is modulated in response to D,L-endopeptidase activity (increased two-fold to compensate for reduced activity in the absence of CwlO or FtsE-FtsX, decreased upon overexpression of LytE) (WalR) PubMed
  • induced at high temperature (SigI, WalR) PubMed
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    Biological materials

    Mutant

  • 1A790 ( lytE::cat), PubMed, available at BGSC
  • 1A792 ( lytE::cat), PubMed, available at BGSC
  • 1A1024 ( lytE::spec), PubMed, available at BGSC
  • BKE09420 (lytE::erm, available in the BGSC and in Jörg Stülke's lab) PubMed
  • BKE09420 (ΔlytE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
  • BKK09420 (ΔlytE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
  • FLAG-tag construct

  • GP2020 lytE-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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