darA [2019-01-08 13:32:40]

c-di-AMP-binding PII-like protein

locus: BSU00290

pI: 6.09

mw: 11.83 kDa

protein length: 109 aa

gene length: 327 bp

function: unknown

product: c-di-AMP-binding PII-like protein

synonyms: yaaQ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 39,871 → 40,200

The protein

Effectors of protein activity

• binds c-di-AMP [Pubmed|25433025]

Structure

• [PDB|4RLE] (the ''B. subtilis'' protein in complex with c-di-AMP) [Pubmed|25433025]

Expression and Regulation

Operons

• genes: [gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
  description: [Pubmed|22383849]

• genes: [gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
  description: [Pubmed|22383849]

  regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase [Pubmed|23490197]

• genes: [gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B897 (yaaQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1896 NBRP B. subtilis, Japan]
• GP1712 (''[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]
• GP1713 (''[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]''::''cat''), available in [SW|Jörg Stülke]'s lab
• GP2406 (''[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2415 (''[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]''::''spc''), available in [SW|Jörg Stülke]'s lab
• GP2497 (''[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]''::''tet''), available in [SW|Jörg Stülke]'s lab
• BKE00290 (Δ[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA
• BKK00290 (Δ[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA

Expression vectors

• pGP2797: expression of ''darA'' (native RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
• pGP3002: expression of ''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
• pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]
• pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jörg Stülke]'s lab
• pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
• pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Jörg Stülke]'s lab

References

Reviews

• 25869574

Original publications

• 22383849,25433025