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ylmE [2019-03-25 11:56:00]
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ylmE [2019-03-25 11:56:00]

enzyme involved in controlling the availability of coenzyme A
locus
BSU15380
pI
5.55
mw
25.56 kDa
protein length
230 aa Sequence Blast
gene length
693 bp Sequence Blast
function
control of the CoA pool
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,610,170 1,610,862

    The protein

    Catalyzed reaction/ biological activity

  • while the protein was described as an amino acid racemase important for biofilm formation [Pubmed|20431016], no racemase activity was detected in ''in vitro'' assays with a functionally active protein [Pubmed|24097949]

    • Protein family

  • UPF0001 family (according to Swiss-Prot)

    • [SW|Cofactors]

  • pyridoxal phosphate [Pubmed|24097949]

    • Structure

  • [PDB|1W8G] (the structure of YggS from ''E. coli'' 33% identity, 61% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
    description
    [Pubmed|16420366]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
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    Biological materials

    Mutant

  • MGNA-B363 (ylmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1362 NBRP B. subtilis, Japan]
  • BKE15380 ([gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACA
  • BKK15380 ([gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACA

    • References

  • 14651647,16420366,24097949,26872910