ylmE [2017-8-11 16:0:3]

enzyme involved in controlling the availability of coenzyme A

locus: BSU15380

pI: 5.55

mw: 25.56 kDa

protein length: 230 aa

gene length: 690 bp

function: control of the CoA pool

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,610,170 → 1,610,862

The protein

Catalyzed reaction/ biological activity

• while the protein was described as an amino acid racemase important for biofilm formation [Pubmed|20431016], no racemase activity was detected in ''in vitro'' assays with a functionally active protein [Pubmed|24097949]

Protein family

• UPF0001 family (according to Swiss-Prot)

[SW|Cofactors]

• pyridoxal phosphate [Pubmed|24097949]

Structure

• [PDB|1W8G] (the structure of YggS from ''E. coli'' 33% identity, 61% similarity)

Expression and Regulation

Operons

• genes: [gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
  description: [Pubmed|16420366]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-B363 (ylmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1362 NBRP B. subtilis, Japan]

References

• 14651647,16420366,24097949,26872910