SubtiBank SubtiBank
ylmE
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ylmE

pyridoxal phosphate-binding protein, involved in controlling the availability of coenzyme A
Locus
BSU_15380
Isoelectric point
5.55
Molecular weight
25.56 kDa
Protein length
230 aa Sequence Blast
Gene length
693 bp Sequence Blast
Function
control of the CoA pool
Product
pyridoxal phosphate-binding protein
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,610,170 1,610,862

The protein

Catalyzed reaction/ biological activity

  • while the protein was described as an amino acid racemase important for biofilm formation PubMed, no racemase activity was detected in in vitro assays with a functionally active protein PubMed
  • Protein family

  • pyridoxal phosphate-binding protein YggS/PROSC family (single member, according to UniProt)
  • Cofactors

  • pyridoxal phosphate PubMed
  • Structure

  • 1W8G (the structure of YggS from E. coli 33% identity, 61% similarity)
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon
  • Regulation

  • repressed under conditions that trigger sporulation (Spo0A) PubMed
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    Biological materials

    Mutant

  • MGNA-B363 (ylmE::erm), available at the NBRP B. subtilis, Japan
  • BKE15380 (ylmE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACA
  • BKK15380 (ylmE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACA
  • References

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