pucK [2018-02-11T13:16:17.000Z]

uric acid permease

locus: BSU32440

pI: 8.35

mw: 44.70 kDa

protein length: 430 aa

gene length: 1290 bp

function: purine utilization

product: uric acid permease

essential: no

synonyms: yunK

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,331,857 → 3,333,149

The protein

Protein family

• xanthine/uracil permease family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|PbuX], [protein|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|PucJ]

Structure

• [PDB|3QE7] (E. coli uracil transporter, 28% identity) [pubmed|21423164]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
  description: [Pubmed|12029039]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

• genes: [gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]-[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: auto-repression, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

Biological materials

Mutant

• MGNA-A936 (yunK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/936 NBRP B. subtilis, Japan]
• BKE32440 (Δ[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT, downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT
• BKK32440 (Δ[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT, downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT

References

• 11344136,25755103,12823818,12029039,21423164