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ydaD [2019-04-16 13:34:48]
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ydaD [2019-04-16 13:34:48]

general stress protein, similar to alcohol dehydrogenase
locus
BSU04190
pI
4.78
mw
30.93 kDa
protein length
286 aa Sequence Blast
gene length
861 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    471,709 472,569

    The protein

    Protein family

  • short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF], [protein|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|BacC]
  • [protein|439B468A13137000FB42E9389391CB4986FFED84|FabG]:
  • [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|YkvO]:
  • [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC]:
  • [protein|739B228743BC1FE9E6888E999BC0E4F615E36F9E|YhxD]:
  • [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF]:
  • [protein|CA4597C6253CCF7D7954686A30AF041808BDF8E5|Gdh]:
  • [protein|D9D1FCBFC62F93CAA34DF61A784406F4E9EE6768|YjdA]:
  • [protein|FBBDE1E058223D5E8CEB07CAA50940A329C809E4|KduD]:
  • [protein|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|BacC] (34.3%), [protein|439B468A13137000FB42E9389391CB4986FFED84|FabG] (31.1%), [protein|FBBDE1E058223D5E8CEB07CAA50940A329C809E4|KduD] (36.7%), [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF]

    • Structure

  • [PDB|3I30] (from ''Bacillus Anthracis'', 61% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]-[gene|DDE975DA2E13759B0DB8B6D219760C2E2C6032FB|ydaE]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|15805528,10220166], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|15805528]
    • view in new tab

    genes
    [gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]-[gene|DDE975DA2E13759B0DB8B6D219760C2E2C6032FB|ydaE]-[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
    description
    [Pubmed|10220166]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528,10220166], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C086 (ydaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2084 NBRP B. subtilis, Japan]
  • BKE04190 ([gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATTCTCCTCCTTT, downstream forward: _UP4_ACGACATAAGAGGGAGTGAG
  • BKK04190 ([gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATTCTCCTCCTTT, downstream forward: _UP4_ACGACATAAGAGGGAGTGAG

    • References

  • 10220166,15805528,23033921