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galE
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galE

UDP glucose 4-epimerase
Locus
BSU_38860
Isoelectric point
4.78
Molecular weight
36.85 kDa
Protein length
339 aa Sequence Blast
Gene length
1020 bp Sequence Blast
Function
galactose utilization
Product
UDP glucose 4-epimerase
Essential
no
E.C.
5.1.3.2
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,989,948 3,990,967

Phenotypes of a mutant

  • smooth colonies on MsGG medium, no biofilm formation in the absence of externally provided galactose PubMed
  • the mutant is sensitive to exogenous galactose due to the accumulation of toxic UDP-galactose, this sensitivity can be suppressed by the inactivation of galK (no formation of the toxic UDP-galactose) or of sinR (constitutive expression of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO results in detoxification of UDP-galactose) PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • UDP-α-D-glucose --> UDP-α-D-galactose (according to UniProt)
  • Protein family

    Structure

  • 1LRL (from Escherichia coli Y299C mutant, 57% identity, 71% similarity) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
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    Biological materials

    Mutant

  • MGNA-B736 (galE::erm), available at the NBRP B. subtilis, Japan
  • BKE38860 (galE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA
  • BKK38860 (galE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA
  • References

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