ycsI

unknown

locus: BSU_04070

pI: 6.41

mw: 28.68 kDa

protein length: 257 aa

gene length: 774 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 459,049 459,822

The protein

Protein family

• D-glutamate cyclase family (single member, according to UniProt)

Structure

• [PDB|3DB9] (from Agrobacterium tumefaciens, 57% identity)

Expression and Regulation

Operons

• genes: [gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]-[gene|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG]-[gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]-[gene|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB]-[gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]-[gene|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]-[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
  description: [Pubmed|9334321]

  regulatory mechanism

  • [protein|7DA9A79876C546B78B716A64706A3A3716018C2E|KipR]: repression, [Pubmed|9334321], in [regulon|7DA9A79876C546B78B716A64706A3A3716018C2E|KipR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|9334321], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • induced in the presence of 5-oxoproline [pubmed|28830929]

Biological materials

Mutant

• MGNA-C023 (ycsI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2021 NBRP B. subtilis, Japan]
• BKE04070 ([gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTTAATTGCTCCA, downstream forward: _UP4_TAGCCTCTCGTCATCCTGAT
• BKK04070 ([gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTTAATTGCTCCA, downstream forward: _UP4_TAGCCTCTCGTCATCCTGAT

References

• 9334321,25755103