nprE

extracellular neutral protease B

Locus: BSU_14700

Isoelectric point: 7.48

Molecular weight: 56.35 kDa

Protein length: 521 aa

Gene length: 1566 bp

Function: degradation of proteins

Product: extracellular neutral protease B

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of proteins
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Extracellular feeding proteases
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,540,036 1,541,601

The protein

Catalyzed reaction/ biological activity

• Similar, but not identical, to that of thermolysin (according to UniProt)

Protein family

• peptidase M4 family (with NprB, according to UniProt)

Paralogous protein(s)

• NprB

Structure

• 1NPC (of Bacillus cereus)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: nprE
  Description: PubMed

  Regulatory mechanism

  • ScoC: repression, PubMed, in ScoC regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed during exponential growth (ScoC) PubMed

  Additional information

  • in a triple abrB codY scoC mutant, expression occurs during exponential growth PubMed

Biological materials

Mutant

• KO7 (nprE aprE epr mpr nprB vpr bpr), available as BGSC 1A1133
• BKE14700 (nprE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAATCCCCCTTTTT, downstream forward: _UP4_TAATATTAGGAAAAGCCTGA
• BKK14700 (nprE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAATCCCCCTTTTT, downstream forward: _UP4_TAATATTAGGAAAAGCCTGA

References

Reviews

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Original publications

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