nprE

extracellular neutral protease B

locus: BSU_14700

pI: 7.48

mw: 56.35 kDa

protein length: 521 aa

gene length: 1566 bp

function: degradation of proteins

product: extracellular neutral protease B

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,540,036 1,541,601

The protein

Catalyzed reaction/ biological activity

• Similar, but not identical, to that of thermolysin (according to UniProt)

Protein family

• peptidase M4 family (with [protein|57139471EB143F6DCCF092DAD0FBB43B0D50D948|NprB], according to UniProt)

Paralogous protein(s)

• [protein|57139471EB143F6DCCF092DAD0FBB43B0D50D948|NprB]

Structure

• [PDB|1NPC] (of ''Bacillus cereus'')

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|nprE]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|1906467], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|26728191], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed during exponential growth ([protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]) [Pubmed|1906467]

  additional information

  • in a triple ''[SW|abrB] [SW|codY] [SW|scoC]'' mutant, expression occurs during exponential growth [Pubmed|26728191]

Biological materials

Mutant

• KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
• BKE14700 ([gene|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|nprE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAATCCCCCTTTTT, downstream forward: _UP4_TAATATTAGGAAAAGCCTGA
• BKK14700 ([gene|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|nprE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAATCCCCCTTTTT, downstream forward: _UP4_TAATATTAGGAAAAGCCTGA

References

Reviews

• 20735481

Original publications

• 1906467,18957862,20817675,24115457,26728191