aag [2017-07-24 15:09:56]

hypoxanthine-DNA glycosylase, general stress protein, required for protection against paraquat stress

locus: BSU38620

pI: 6.12

mw: 21.98 kDa

protein length: 196 aa

gene length: 588 bp

function: DNA repair, survival of stress conditions

product: hypoxanthine-DNA glycosylase

essential: no

synonyms: yxlJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 3,964,278 → 3,964,868

Phenotypes of a mutant

• increased sensitivity of sporulating cells to the genotoxic effects of MMS [Pubmed|27698084]

The protein

Catalyzed reaction/ biological activity

• removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates [Pubmed|14729667]
• protects sporulating cells from cytotoxic and genotoxic effects of MMS [Pubmed|27698084]

Protein family

• DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases

Structure

• [PDB|3QI5] (the human enzyme in complex with DNA, 33% identity) [pubmed|21349833]

[SW|Localization]

• forespore [Pubmed|27698084]

Expression and Regulation

Operons

• genes: [gene|305DCA94A69BCF64E377F142722A035871A3B824|aag]-[gene|9A30DA670D03D63C59DEB54EA7BD6DCEDDBD561A|yxzF]
  description: [Pubmed|8990303]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|27698084], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed late during [SW|sporulation] in the forespore [SW|SigG] [Pubmed|27698084]

Biological materials

Mutant

• MGNA-B756 (yxlJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1755 NBRP B. subtilis, Japan]

References

Reviews

• 22933559

Original publications

• 15805528,14729667,22582280,27698084,21349833