aag [2017-07-24 15:09:56]

hypoxanthine-DNA glycosylase, general stress protein, required for protection against paraquat stress

Locus: BSU38620

Isoelectric point: 6.12

Molecular weight: 21.98 kDa

Protein length: 196 aa

Gene length: 588 bp

Function: DNA repair, survival of stress conditions

Product: hypoxanthine-DNA glycosylase

Essential: no

Synonyms: yxlJ

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination/ based on similarity
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 3,964,278 → 3,964,868

Phenotypes of a mutant

• increased sensitivity of sporulating cells to the genotoxic effects of MMS PubMed

The protein

Catalyzed reaction/ biological activity

• removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed
• protects sporulating cells from cytotoxic and genotoxic effects of MMS PubMed

Protein family

• DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases

Structure

• 3QI5 (the human enzyme in complex with DNA, 33% identity) PubMed

Localization

• forespore PubMed

Expression and Regulation

Operons

• Genes: aag-yxzF
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expressed late during sporulation in the forespore SigG PubMed

Biological materials

Mutant

• MGNA-B756 (yxlJ::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original publications

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