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efp [2017-09-16 17:08:28]
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efp [2017-09-16 17:08:28]

elongation factor P, important for the proteins containing three or more consecutive proline residues
locus
BSU24450
pI
4.84
mw
20.31 kDa
protein length
185 aa Sequence Blast
gene length
555 bp Sequence Blast
function
translation
product
elongation factor P
essential
no
synonyms
yqhU,yqgF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]

    • Gene

    Coordinates
    2,538,115 → 2,538,672

    Phenotypes of a mutant

  • impaired swarming motility [Pubmed|15066026,27002156]
  • inactivation of ''[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]'' reduces sporulation efficiency to 1% that of wild type cells [Pubmed|26735940]

    • The protein

    Protein family

  • transpeptidase family (according to Swiss-Prot) elongation factor P family (according to Swiss-Prot)

    • Modification

  • carries a 5-aminopentanol moiety at Lys-32, this modification is essential for activity [Pubmed|27002156]

    • Structure

  • [PDB|1YBY] (Efp from ''Clostridium thermocellum'')

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), cell membrane (according to Swiss-Prot)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|449EEBF33F52B461F244331ACD140E68CF64EE7C|yqhR]-[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-C464 (efp::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2462 NBRP B. subtilis, Japan]
  • BKE24450 (''[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • BKE24450 (Δ[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU24450 BGSC], upstream reverse: 
    _UP1_CATGTTTAATGTCCTCCTAT
    , downstream forward: 
    _UP4_TAGAAAGAAAAAAAGAAGTC
  • BKK24450 (Δ[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU24450 BGSC], upstream reverse: 
    _UP1_CATGTTTAATGTCCTCCTAT
    , downstream forward: 
    _UP4_TAGAAAGAAAAAAAGAAGTC

    • References

    Reviews

  • 26416626

    • Original publications

  • 15066026,17981983,23239624,23239623,25310979,25144653,27002156,26735940,27216360,26384033,28787546,28765223