efp [2018-04-09 17:08:59]

elongation factor P, important for the Translation of proteins containing three or more consecutive proline residues

Locus: BSU24450

Isoelectric point: 4.84

Molecular weight: 20.31 kDa

Protein length: 185 aa

Gene length: 555 bp

Function: Translation

Product: elongation factor P

Essential: no

Synonyms: yqhU,yqgF

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming

Gene

Coordinates: 2,538,115 → 2,538,672

Phenotypes of a mutant

• impaired swarming motility PubMed
• inactivation of efp reduces sporulation efficiency to 1% that of wild type cells PubMed

The protein

Protein family

• transpeptidase family (according to Swiss-Prot) elongation factor P family (according to Swiss-Prot)

Modification

• carries a 5-aminopentanol moiety at Lys-32, this modification is essential for activity PubMed

Structure

• 1YBY (Efp from Clostridium thermocellum)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: yqhR-yqhS-papA-efp
  Description: PubMed

• Genes: yqhS-papA-efp
  Description: PubMed

Biological materials

Mutant

• MGNA-C464 (efp::erm), available at the NBRP B. subtilis, Japan
• BKE24450 (efp::erm, available in the BGSC and in Jörg Stülke's lab) PubMed
• BKE24450 (Δefp::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTAATGTCCTCCTAT, downstream forward: _UP4_TAGAAAGAAAAAAAGAAGTC
• BKK24450 (Δefp::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTAATGTCCTCCTAT, downstream forward: _UP4_TAGAAAGAAAAAAAGAAGTC

References

Reviews

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Original publications

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