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tsaD
required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
function
tRNA modification
product
tRNA modification enzyme
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]Gene
Coordinates
643,258 644,298
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]adenosine37 in tRNA + L-threonylcarbamoyladenylate --> AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA (according to UniProt)Protein family
KAE1 / TsaD family (with [protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB], according to UniProt)Modification
in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE] [pubmed|28890133]Structure
[PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679][SW|Localization]
cytoplasm (according to UniProt)Additional information
[SW|universally conserved protein]Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
view in new tabReferences
Research papers
Publications on the corresponding protein from other species
21183954,19376873,20824107,21619589,21285948,21625506,23056478,23471679,28890133,29741707