yhdU [2018-11-16 16:26:59]

flouride exporter

locus: BSU09600

pI: 9.85

mw: 12.31 kDa

protein length: 118 aa

gene length: 354 bp

function: export of flouride

product: flouride channel

essential: no

synonyms: crcBA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.11|Other exporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,036,953 → 1,037,309

The protein

Protein family

• crcB family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|BD1C4D9E6E8960DF77EE1571A637929C2D8C4A28|YhdV]

Structure

• [PDB|5A40] (from Bordetella pertussis, 41% identity, aa 7 ... 87) [pubmed|26344196]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW]-[gene|BD1C4D9E6E8960DF77EE1571A637929C2D8C4A28|yhdV]-[gene|2F86A2FF36ED00DB0A9A91B064EBD095F5E759ED|fluC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B486 (yhdU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1485 NBRP B. subtilis, Japan]
• BKE09600 (Δ[gene|2F86A2FF36ED00DB0A9A91B064EBD095F5E759ED|fluC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTATGAAAATACCCGCTA, downstream forward: _UP4_ATCACAATATAAAAAAACCT
• BKK09600 (Δ[gene|2F86A2FF36ED00DB0A9A91B064EBD095F5E759ED|fluC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTATGAAAATACCCGCTA, downstream forward: _UP4_ATCACAATATAAAAAAACCT

References

Reviews

• 28514705

Research papers

• 26344196,23991286,30430725