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ntdA [2020-09-09 14:31:43]
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ntdA [2020-09-09 14:31:43]

pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
locus
BSU_10550
pI
5.97
mw
49.98 kDa
protein length
441 aa Sequence Blast
gene length
1326 bp Sequence Blast
function
synthesis of the antibiotic kanosamine
product
pyridoxal phosphate-dependent
essential
no
synonyms
yhjL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    1,128,286 1,129,611

    The protein

    Catalyzed reaction/ biological activity

  • 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate [Pubmed|23586652]

    • Protein family

  • DegT/DnrJ/EryC1 family (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|EpsN] and [protein|38DBA751456A0C0EC5D028939ADB8860ADA28F94|SpsC], according to UniProt)

    • [SW|Cofactors]

  • pyridoxalphosphate [Pubmed|23586652]

    • Structure

  • [PDB|4K2I] [Pubmed|24097983]

    • Expression and Regulation

    Operons

    genes
    [gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]
    description
    [Pubmed|17056753]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14612444], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR]: activation, in [regulon|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR regulon]

    • regulation

  • induced by 3,3'-neotrehalosadiamine ([protein|search|NtdR]) [Pubmed|14612444]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A725 (yhjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/725 NBRP B. subtilis, Japan]
  • BKE10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
  • BKK10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA

    • References

    Reviews

  • 21512256

    • Original publications

  • 19087206,19342798,17056753,14612444,23586652,24097983