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ntdA [2019-05-10 10:10:52]
pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
function
synthesis of the antibiotic kanosamine
product
pyridoxal phosphate-dependent
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]Gene
Coordinates
1,128,286 1,129,611
The protein
Catalyzed reaction/ biological activity
3-oxo-d-glucose-6-phosphate + glutamate - kanosamine-6-phosphate [Pubmed|23586652]Protein family
degT/dnrJ/eryC1 family (according to Swiss-Prot)[SW|Cofactors]
pyridoxalphosphate [Pubmed|23586652]Structure
[PDB|4K2I] [Pubmed|24097983]Expression and Regulation
Operons
genes
[gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]
description
[Pubmed|17056753]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14612444], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR]: activation, in [regulon|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR regulon]regulation
induced by 3,3'-neotrehalosadiamine ([protein|search|NtdR]) [Pubmed|14612444]view in new tabBiological materials
Mutant
MGNA-A725 (yhjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/725 NBRP B. subtilis, Japan]BKE10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGABKK10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGAReferences
Reviews
Original publications
19087206,19342798,17056753,14612444,23586652,24097983