ntdA

pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

locus: BSU_10550

pI: 5.97

mw: 49.98 kDa

protein length: 441 aa

gene length: 1326 bp

function: synthesis of the antibiotic kanosamine

product: pyridoxal phosphate-dependent

essential: no

synonyms: yhjL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,128,286 1,129,611

The protein

Catalyzed reaction/ biological activity

• 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate [Pubmed|23586652]

Protein family

• DegT/DnrJ/EryC1 family (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|EpsN] and [protein|38DBA751456A0C0EC5D028939ADB8860ADA28F94|SpsC], according to UniProt)

[SW|Cofactors]

• pyridoxalphosphate [Pubmed|23586652]

Structure

• [PDB|4K2I] [Pubmed|24097983]

Expression and Regulation

Operons

• genes: [gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]
  description: [Pubmed|17056753]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14612444], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR]: activation, in [regulon|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR regulon]

  regulation

  • induced by 3,3'-neotrehalosadiamine ([protein|search|NtdR]) [Pubmed|14612444]

Biological materials

Mutant

• MGNA-A725 (yhjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/725 NBRP B. subtilis, Japan]
• BKE10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
• BKK10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA

References

Reviews

• 21512256

Original publications

• 19087206,19342798,17056753,14612444,23586652,24097983,32786294