ntdA

pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

Locus: BSU_10550

Isoelectric point: 5.97

Molecular weight: 49.98 kDa

Protein length: 441 aa

Gene length: 1326 bp

Function: synthesis of the antibiotic kanosamine

Product: pyridoxal phosphate-dependent

Essential: no

Synonyms: yhjL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,128,286 1,129,611

The protein

Catalyzed reaction/ biological activity

• 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate PubMed

Protein family

• DegT/DnrJ/EryC1 family (with EpsN and SpsC, according to UniProt)

Cofactors

• pyridoxalphosphate PubMed

Structure

• 4K2I PubMed

Expression and Regulation

Operons

• Genes: ntdA-ntdB-ntdC-glcP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • NtdR: activation, in NtdR regulon

  Regulation

  • induced by 3,3'-neotrehalosadiamine (NtdR) PubMed

Biological materials

Mutant

• MGNA-A725 (yhjL::erm), available at the NBRP B. subtilis, Japan
• BKE10550 (ntdA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
• BKK10550 (ntdA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAACAGTACCTCCAATG, downstream forward: _UP4_TTTCATGTTATTAAGCAAGA

References

Reviews

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Original publications

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