trmU [Mon Jan 18 2016 18:07:55 GMT+0100 (CET)]

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

locus: BSU27500

pI: 4.00

mw: 42.00 kDa

function: tRNA modification

product: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

essential: yes

synonyms: yrrA, mnmA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|stringent response]

Gene

Coordinates on the chromosome (coding sequence): 2,809,413 -> 2,810,528

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|search|YrvO]) [Pubmed|25825430]

Protein family

• mnmA/TRMU family (according to Swiss-Prot)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

[SW|Interactions]

• [protein|search|YrvO]-[protein|search|TrmU] [Pubmed|25825430]

Expression and Regulation

Operon

• ''[protein|search|cymR]-[protein|search|yrvO]-[protein|search|trmU]'' [Pubmed|16885442]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|16885442]

Regulation

• [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

References

• 16885442,11948165,25825430