trmU

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

Locus: BSU_27500

Isoelectric point: 4.73

Molecular weight: 42.20 kDa

Protein length: 371 aa

Gene length: 1116 bp

Function: tRNA modification

Product: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

Essential: yes

E.C.: 2.8.1.-

Synonyms: yrrA,mnmA

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
• Groups of genes → Essential genes

Gene

Coordinates: 2,809,413 2,810,528

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with YrvO) PubMed
• AH₂ + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine³⁴ in tRNA --> 2-thiouridine³⁴ in tRNA + A + AMP + diphosphate + H⁺ + L-cysteinyl-[protein] (according to UniProt)

Protein family

• MnmA/TRMU family (single member, according to UniProt)

Structure

• 2HMA (from Streptococcus pneumoniae, 66% identity)

Localization

• cytoplasm(according to UniProt)

Expression and Regulation

Operons

• Genes: cymR-yrvO-trmU-yrrB-recD2
  Description: PubMed

• Genes: cymR-yrvO-trmU
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

References

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