trmU

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

locus: BSU_27500

pI: 4.73

mw: 42.20 kDa

protein length: 371 aa

gene length: 1116 bp

function: tRNA modification

product: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

essential: yes

ec: 2.8.1.-

synonyms: yrrA,mnmA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,809,413 2,810,528

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO]) [Pubmed|25825430]
• AH₂ + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine³⁴ in tRNA --> 2-thiouridine³⁴ in tRNA + A + AMP + diphosphate + H⁺ + L-cysteinyl-[protein] (according to UniProt)

Protein family

• MnmA/TRMU family (single member, according to UniProt)

Structure

• [PDB|2HMA] (from ''Streptococcus pneumoniae'', 66% identity)

[SW|Localization]

• cytoplasm(according to UniProt)

Expression and Regulation

Operons

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
  description: [Pubmed|22383849]

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
  description: [Pubmed|16885442]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16885442], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

References

• 16885442,11948165,25825430